Instrumental Methods Of Analysis

Unit 1

UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert's law, Derivation and deviations.

Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectorsPhoto tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications - Spectrophotometric titrations, Single component and multi component analysis

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Unit 2

IR Spectroscopy

Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations

Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications

Flame Photometry

Principle, interferences, instrumentation and applications

Atomic absorption spectroscopy

Principle, interferences, instrumentation and applications

Nepheloturbidometry

Principle, instrumentation and applications

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Unit 3

Introduction to chromatography

Adsorption and partition column chromatography

Methodology, advantages, disadvantages and applications.

Thin layer chromatography

Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.

Paper chromatography

Introduction, methodology, development techniques, advantages, disadvantages and applications

Electrophoresis

Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications

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Unit 4

Gas chromatography

Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications

High performance liquid chromatography (HPLC)

Introduction, theory, instrumentation, advantages and applications.

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Unit 5

Ion exchange chromatography

Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications

Gel chromatography

Introduction, theory, instrumentation and applications

Affinity chromatography

Introduction, theory, instrumentation and applications